21 G x .75 in. DO NOT SHAKE OR MIX VIGOROUSLY. The tourniquet will be applied 3-4 inches above the selected puncture site and will not .
PDF Phlebotomy Venipuncture Procedure (LTR34351) 3. Specimens should not be obtained from the arm on the same side as a mastectomy.
[16] In developing countries, it is still common to draw blood using a syringe or syringes. My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. (6 J. Select the collection site and proceed as routine phlebotomy. It is not recommended that blood be drawn from the feet .The Providers permission is required to draw from this site. 10.
Specimen Collection Procedure - PERFORMING A VENIPUNCTURE | Akron The Importance of Early Learning for Growing Kids. 0000034815 00000 n
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7.1.4.5 Refer to BD Vacutainer Venous Blood Collection Tube Guide for complete listing and description: This is to prevent cross contamination by the tube additives that could lead to erroneous results. The BD Vacutainer blood transfer device is designed with healthcare workers' safety in mind. 0000015000 00000 n
Whenever a Large amount of blood is required for the tests, venipuncture is done to obtain the bloodsample. Take the syringe in right hand, holding with the index finger against the base of the needle, keeping the bevel of the needle to the upper side and push firmly & steadily without any hesitation into the center of the vein. /Size[255] 0000009793 00000 n
endobj
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Clean the puncture site by making a smooth circular pass over the site with the 70%alcohol pad, moving in an outward spiral from the zone of penetration. 2 0 obj Firstly ensure that the patient should be in the comfortable position. 0000014709 00000 n
Do not touch the puncture site after cleaning. BD offers training resources to help improve your clinical practices as part of our goal of advancing the world of health. Collect blood into the tube using the standard technique for BD Vacutainer Evacuated Blood Collection Tubes (see Venipuncture Technique & Sample Collection section and Prevention of <>
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A sales representive will get in touch with you shortly. 8. 6. 21g or less 3. BD supports the healthcare industry with market-leading products and services that aim to improve care while lowering costs. Vacutainer Plasma Preparation Tubes (PPT) BD. 0000011261 00000 n
tubing and no luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. 0000020236 00000 n
D. Holding the needle in line with the vein, use a quick, small thrust to penetrate the skin and enter the vein in one smooth motion. 9. /Filter[/FlateDecode] Arthroscopy Trays; Circumcision Trays; Ophthalmic Trays; Shoulder Trays; Stockinettes; Surgical Drapes endobj
Avoid drawing blood from a hematoma. This procedure establishes criteria for the proper collection of blood specimens. BD Vacutainer Safety-Lok Blood Collection Set with 12 in. BD promotes clinical excellence by providing various resources on best practices, clinical innovations and industry trends in healthcare. trailer BD Vacutainer plastic tubes offer a safe method of blood collection and reduce the potential for tube breakage and specimen spillage, thereby reducing the potential for exposure to bloodborne pathogens. Lay the arm of the patient on the table.
Guide to using the BD Vacutainer Blood Collection Set - YouTube 1. The needle which pierces the tubes can carry additives from one tube into the next, so the sequence is standardized so that any cross-contamination of additives will not affect laboratory results.[7]. 25 G x .75 in. Hi, Im the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. BD Vacutainer PPT Plasma Preparation Tubes. xref endobj
BD Vacutainer Safety-Lok Blood Collection Set with 7 in. %
Tubes are available with a safety-engineered stopper, with a variety of labeling options and draw volumes.
BD Vacutainer Venous Blood Collection Tube Whole Blood Tube ACD %PDF-1.4
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Dispose of the syringe and needle as a unit into an appropriate sharps container. tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Individually packaged 70% isopropyl alcohol wipes. Mk 3. Blood Collection Process: Venipuncture Venipuncture Procedure PDF ROUTINE VENIPUNCTURE GUIDELINES MATERIALS 1. X mmms?mmnM These are available from Becton Dickinson (BD). The BD Vacutainer blood transfer device was designed with healthcare workers' safety in mind. This blood will be used for staining. The BD Vacutainer PST Tube still provides the convenience of gel separation with the added advantage of improved turnaround times. A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. 13. If anticoagulant is used, gently shake the vials for proper mixing of the blood with the anticoagulant. If the child is old enough, collect blood as in an adult. transfer device to fill vacutainer tubes, allow the vacuum to pull the blood. 23 G x .75 in.
Platelet Activation - BD Biosciences 2. Now decide the amount of blood required and select the appropriate vials or containers according to the tests to be done. Release the tourniquet. 0000218054 00000 n
Wash hands in warm, running water with a appropriate hand washing product. Carefully Remove the needle from the syringe and gently expelthe blood into the appropriate vial or container. /Size[2] 3.
SOP 2.5 Blood Collection - Venipuncture - National Institutes of Health 3 0 obj
SOP COLLECTION OF BLOOD - National Institutes of Health 21 G x .75 in. Blood Collection Tubes. BD Vacutainer Safety-Lok Blood Collection Set with 7 in. Remove plastic cap over needle and hold bevel up. Do not attempt a venipuncture more than twice. PRESS sample blood tube(s) on to vacutainer needle. Subscribe To Our Newsletter To Get All The Updates Right in Your Inbox, BLOOD COLLECTION BY VENIPUNCTURE SYRINGE AND VACUTAINER METHOD, REQUIREMENTS FOR THE BLOOD COLLECTION BY SYRINGE METHOD, PROCEDURE OF THE BLOOD COLLECTION BY SYRINGE METHOD, REQUIREMENTS FOR THE BLOOD COLLECTION BY VACUTAINER METHOD, PROCEDURE OF BLOOD COLLECTION BY VACUTAINER METHOD. tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Overview The use of a needle to transfer venous blood from a syringe to a blood collection tube or blood culture bottle is both a dangerous procedure and an OSHA-prohibited practice. 0000182441 00000 n
/TR/Identity 8. BD Vacutainer SST Venous Blood Collection Tube Serum Tube Clot Activator / Separator Gel Additive 16 X 100 mm 8.5 mL Red / Gray Mottled Conventional Closure Plastic Tube
Procedure for Serum Processing from Whole Blood Origination Date: Total 13x75 mm 3.0 mL BD Vacutainer plastic P700 plasma tube. endobj
We host and take part in events that excel in advancing the world of health. I. /Subtype/Image Needles and hubs are single use and are disposed of in an appropriate sharps container as one unit. The single-use device reduces the risk of transfer-related injuries, while maintaining the specimen integrity required for accurate results. But there should not be any air present in the syringe at the time of blood collection. Size - 13mm x 75mm. E. Holding the hub securely, insert the first vacutainer tube following proper order of draw into the large end of the hub penetrating the stopper. (adsbygoogle = window.adsbygoogle || []).push({}); Paramedics World was founded in 2017 by Sahil Batra, with the sake to provide medical and paramedical information and reference meterial to the students and the professionals. Now Assemble the vacutainer holder & needle by inserting short end of the vacutainer needle which is covered with the rubber coating into the threaded end of the holder. It is also offered with a preattached holder to add convenience and help ensure OSHA single-use holder compliance. 0000036227 00000 n
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This procedure is one example of a variety of methods that can be used to activate platelets. Needles and hubs are single use and are disposed of in . Sharps Disposal Container. BD Vacutainer PST Lithium Heparin Tubes eliminate the need to wait for a clot to form, making it an ideal tube for STAT procedures, as well as for patients receiving anticoagulant therapy. The Veterinary Surgeon shall enter the swab card number on each of the vacutainer tubes; 3. %PDF-1.5
Used for chemistry and reference tests. Your email address will not be published. Gently invert tubes containing an additive 5-8 times. The Blog is basically devoted to the Paramedical personnels who risk their life to save the life of other peoples. 3. BD's collection of literature on industry and on our offerings gives you information you can use to continue striving for excellence. 12. If commercially available tubes are to be used, the researcher should use the red topped tubes. Screw it tightly using a clockwise motion. 0000099961 00000 n
If using a needle. SST PST EDT A S . <>
Gloves are to be worn during all phlebotomies, and changed between patient collections. "kB#i]#}= -XIYFIO^qpr;aR Ask the patient to state their name and date of birth. }J]UV\e|}*>O\,qTC\q\/'rYR.>v
6EoO^jUqU],?B`|9N]7K!A*K i6]IRwLvHH&UnbQ4 ,oluQzO#L_n(^}Qa$}T-o?%RrcSI+VBIQhUD*n5g1k.Tkmm/u|gAz-+)m[dmNjKU Only BD provides a full offering of blood collection tubes in both glass and plastic. Now Instruct the patient to relax and unclench the fist and release the tourniquet after the required amount of blood is obtained as per the tests to be done and prepare to withdraw the needle. /Filter[/FlateDecode] 10. 4. 6. /Domain[0 1] 0000182371 00000 n
2. UF Health Pathology Laboratories Nontuberculosis Mycobacteria Laboratory, This page uses Google Analytics (Google Privacy Policy). 0000010451 00000 n
Identify the patient, two forms of active identification are required. With your left hand, slightlypull back the piston till the required amount of blood is obtained into the syringe. 2 0 obj
/BitsPerComponent 8 All rights reserved. 11. Pull the skin tight with your thumb or index finger just below the puncture site. A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. If hands are not visibly contaminated a commercial foaming hand wash product may be used before and after each patient collection. 0000009374 00000 n
1 0 obj
Instruct the patient to apply the pressure over the wound until the blood ceases. 4. << !J"~9iW
Blood Collection Tube Serum Tube Clot Activator 1. 0000010686 00000 n
/Height 64 How to buy Reach out to our team for ordering information. 25 G x .75 in. Single use, disposable, latex-free tourniquets. #labtechniciancourse #laboratory #bloodcollection #labtech #bloodsample #bloodbank #collection #trending #viral #viralvideo #video #metropolis #hospital /Length 13388 a BD Vacutainer Safety-Lok Blood Collection Set. If using a blood. PROCEDURE OF BLOOD COLLECTION BY VACUTAINER METHOD Firstly ensure that the patient should be in the comfortable position. >>
Blood Collection Process: Venipuncture - University of Florida Draw the desired amount of blood by pulling back slowly on the syringe stopper. 6. The vacuum tubes are designed to draw a predetermined volume of blood. 2023 BD. 6. /Filter[/FlateDecode] Overview The BD Vacutainer Safety-Lok blood collection set is simple, easy to use and safety engineered. With the right hand, Grasp the vacutainer unit (Vacutainer holder+needle) and remove the translucent plastic cover from the needle. Begin with a quality specimen for quality results. <>>>
The tube must be labeled before leaving the patient.
Vacutainer Blood Collection at Thomas Scientific A Blood test is prescribed in most of the blood-related disorders, metabolic disorders and in various infections as well as for the Regular health Check-ups. [1], Vacutainer tubes were invented by Joseph Kleiner in 1949. If a tourniquet is used for preliminary vein selection, release it and reapply after two minutes. Each tube is topped with a color-coded plastic or rubber cap. *E)\,SDh,M2YdC*6TjM,SU[f+6To>;PcvVB:u{f/>h?p`B9&sMG(14h1>'rbNdZOi;#gnVKy9r!b:/tY"WmU\Kur#6fzo#woW}?`yCrhG1q<>O`yOSsjg1/Kr)S/vE^jf\2Y7)of-y+sowGnE,~(f#(KYD>iVvw=6-'l4?cu~o;v}?=?/5cO?dendstream
Phlebotomy: Background, Indications, Contraindications - Medscape >> Transfer blood drawn into the appropriate tubes as soon as possible using a Blood Transfer Device, as a delay could cause improper coagulation. tubing and no luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. BD VacutainerSafety-Lok blood collection set. BD Vacutainer Safety-Lok Blood Collection Set with 7 in. Disposable, safety needle (21G, 22G, or 21, 23, 25G butterfly needles) B. <>stream
Apply the tourniquet 3-4 inches above the collection site. endobj
The filled tube is then removed and another can be inserted and filled the same way. The use of a needle to transfer venous blood from a syringe to a blood collection tube or blood culture bottle is both a dangerous procedure and an OSHA-prohibited practice. The BD Vacutainer blood transfer device was designed with healthcare workers safety in mind. When the required amount of the blood obtained, carefully pull the tube downwards, off the tube end of the needle and out of the holder. Used primarily for hematology and blood bank testing. This order of draw should be followed whenever multiple tubes are drawn during a single venipuncture. ESG (Environmental, Social, and Governance), Policies, Guidelines and Statements Center, Our live chat is available between the hours of 8.30am - 5.00pm EST, Monday - Friday, BD Original Equipment Manufacturing (OEM), Patient Care Support Across the Continuum. Place the tips of the index and middle fingers of the right hand behind the flange of the vacutainer holder. . 5.
Used by chemistry for glucose testing. <>
Verify that any diet or time restrictions have been met. Collect the blood samples according to the institutional routine clinical procedures. Safety Needles, 22g or less 2. 2 0 obj
PDF BD Vacutainer - Stanford University Reassure the patient that the minimum amount of blood required for testing will be drawn. Notify your supervisor or patients physician if unsuccessful. n. Related Products: Edta Tube. 155 48 stream BD Vacutainer EST tubes may be used as a discard tube, to remove the dead space from the tubing of blood collection sets. Vacutainer BD The BD Vacutainer venous collection consists of the Push Button Blood Collection Set designed to help protect you and your practice from needlestick injuries. 0000014235 00000 n
7. BD Vacutainer Safety-Lok Blood Collection Set with 12 in.
PDF 1. Safety Needles, 22g or less 2. Butterfly needles. 21g or less 3 1. Prior to collection, consult the specific test ordered to determine the proper tube type and specimen volume required. The Central Blood Analysis Laboratory (CBAL) will be responsible for special blood collection and handling protocols as well as training and QC monitoring at the Clinical Centers. 7. 5.4 Collect blood into three (3) 8ml CPT tubes per sample using the standard technique for BD Vacutainer Evacuated Blood Collection Tubes (see Venipuncture Technique & Sample Collection section and Prevention of Backflow section). 1 0000010428 00000 n
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7. Transfer Device 6. 0000015567 00000 n
Venipuncture can be done by two methods:
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BD offers training resources to help improve your clinical practices as part of our goal of advancing the world of health. 0000013645 00000 n
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dqh mYl3(@V"PnIy+`bjj !ER*b#5,~F-0PC nD "a&x8cWglxn1..2\m2i0O All rights reserved. Place a sheathed needle or butterfly on the syringe. /BitsPerSample 8 0000016848 00000 n
6. Vacutainer tube holder 5. C. Pull the skin tight with your thumb or index finger just below the puncture site. 1. ZAJezAqPfI1-4o0]wh1_ea.wWnn;,7\[j@.4cj'DU)ai0A9v}p sh5TK@h %XZE#eCKnL- Refer to the eLab Green Top (plasma): With and without gel, contains lithium heparin. A Vacutainer blood collection tube is a sterile glass or plastic tube with a closure that is evacuated to create a vacuum inside the tube facilitating the draw of a predetermined volume of liquid. These tubes are used primarily for chemistry tests. 4. When bleeding stops, apply a fresh bandage, gauze or tape. All labels must include two identifiers . X
[endstream I am a Medical Lab Tech, a Web Developer and Bibliophiliac. 2. 0000007374 00000 n
If the unit and needle are not held steady while pushing in the tube, the needle may slip out of the vein or puncture the opposing vein wall. A sales representive will get in touch with you shortly. BD promotes clinical excellence by providing various resources on best practices, clinical innovations and industry trends in healthcare. (adsbygoogle = window.adsbygoogle || []).push({});
. 3,4; Whole Blood Activation. Lavender BD Hemogard closure. When bleeding stops, apply a fresh bandage, gauze or tape. The re-use of vacutainer blood tube holders is strictly prohibited by OSHA and BVHS. Bd Vacutainer System Tube Guide Pdf . Transfer Device 6. All rights reserved. 0000003048 00000 n
Blood specimen collection collection accessories Browse all specimen collection products BD Vacutainer gel tubes FAQs General SST and PST questions SST-specific tube questions Contact Sales Contact Support Please note, not all products, services or features of products and services may be available in your local area. Position the patient with the arm extended to form a straight-line form shoulder to wrist.
Vacutainer - Wikipedia At no time may phlebotomists perform venipuncture on an artery. )jVdZV46 The BD Vacutainer one-use holder is compatible with the entire BD Vacutainer blood collection system: the BD Vacutainer Eclipse blood collection needle, the BD Vacutainer Safety-Lok blood collection set, BD Vacutainer push button blood collection sets and BD Vacutainer multiple sample luer adapters. Contact us CUSTOMER SERVICE For product specifications, service, and certificate requests, please call our Customer Service team. This decreases the complexity of steps for mononuclear cell separation, thereby minimizing variability from sample processing. Volume - 3ml. XUwpYfU!%JeU{ B $@ a$44q
hhzgvv7v^{kLfEsK=*D~so1(c5Z0k0P$6!_ Chg$XFUl5 Ask the patient to apply pressure to the gauze for at least 2 minutes. Vacutainers have the advantage of being prepared with additives, allowing easy multi-tube draws, and having a lower chance of hemolysis. Required fields are marked *. Now Assemble the vacutainer holder & needle by inserting short end of the vacutainer needle which is covered with the rubber coating into the threaded end of the holder. >> All other trademarks are the property of their respective owners. 23 G x .75 in. dc0;wb7S\htr[V1F{$N8|N1+P.DRiFn/H&0w[O'PQSw1ete|4$6i&$mS]ZR*6E&;l#Ff6O!Mhl83VYbOSa4L'b:,WF#l!M]-C=I25@I|`3Q+NtW:\$j!epH|U)_li _UGG
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Blood Collection Tubes, Blood Collection Needles and Lancets The one-step, closed-system tube for blood collection, undiluted plasma preparation and transportation offers convenience, safety and high-quality plasma for molecular diagnostic testing. The momentneedle enters the vein, blood flows back into the syringe. We support our products with extensive clinical expertise and educational materials to help you achieve the best possible results. This latex-free, single-use device Compare this item 2 0 obj
Save my name, email, and website in this browser for the next time I comment. tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Blood collection tubes expire because over time the vacuum is lost and blood will not be drawn into the tube when the needle punctures the cap. i$o.-N %0 $e5eC6X} &f V)Z|{as@l G9@(+3`'I'N =I*+L'n. With the left hand, hold the patients arm so that the skin over the vein gets tightened and more prominent. Allow each tube to fill up with the adequate amount of blood required for the lab tests ordered. The amount of air evacuated from the tube predetermines how much blood will fill the tube before blood stops flowing. These additives are typically thin film coatings applied using an ultrasonic nozzle. A sales representive will get in touch with you shortly. 0000015594 00000 n
The Supervisor should be called to assess the patient. endobj 0000011440 00000 n
/Width 63 It is also offered with a preattached holder to add convenience and help ensure OSHA single-use holder compliance.
Blood Collection Tubes. 0000013853 00000 n
Collection of Diagnostic Venous Blood Specimens - Clinical and Laboratory Standards Institute 2017 Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book - Richard A. McPherson . Butterfly needles. 0 Seal-Tight Disposable Air/Water Syringe Tip; Sterile Procedure Trays . use of an incorrect anticoagulant. The Veterinary Surgeon may cleanse the skin of the horse from where the blood sample is to If more than one specimen is needed, push another tube into the holder. 0000002838 00000 n
BD Vacutainer CPT is a fully-closed system for separation of mononuclear cells from whole blood, where cell separation is carried out in the primary blood collection tube. 0000018520 00000 n
Blood collection procedures should be approved by the institutional ethics committee (AWERB, IACUC or equivalent) and detailed in the approved study protocol.
Lavender or Pink Top (plasma): Contains EDTA. tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Blood Collection Tubes. The meanings of the various colors are standardized across manufacturers. The BD EST tubes are plastic tubes and have no additive in them.
PDF VENOUS BLOOD COLLECTION (Venipuncture) - Johns Hopkins University Plasma and Serum Preparation | Thermo Fisher Scientific - US /Type/ExtGState 5. This aid in the prevention of bruising and edema formation. BD Vacutainer Safety-Lok Blood Collection Set with 12 in. and syringe, avoid drawing the plunger back too forcefully. 15. Release tourniquet and collect blood into a labeled ACD VACUTAINER tube. /Type/XObject Immediately apply slight pressure to the gauxe pad over the venipuncture site.. 12. 1 0 obj
<< Avoid probing for the vein. <>/OutputIntents[<>] /Metadata 580 0 R>>
The following order of draw is the approved order as established by CLSI. 10.
PDF Policy Nztr Swabbing Instructions BD Vacutainer SST Venous Blood Collection Tube Serum Tube Clot Activator / Separator Gel Additive 16 X 100 mm 8.5 mL Red / Gray Mottled Conventional Closure Plastic Tube Phlebotomy is a technique in which a needle is temporarily inserted into a vein to provide venous access for venous blood sampling. tubing and pre-attached holder in a fully sterilized package, offering an integrated device that may simplify the phlebotomy procedure. Extensive scarring or healed burn areas should be avoided. 0000001256 00000 n
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This information must match the requisition. Ensure that the collection tube is completely pushed onto the back of the needle in the hub. [5][6][7], The term order of draw refers to the sequence in which tubes should be filled. The plastic tube version, known as Vacutainer PLUS, was developed at B-D in the early 1990s by E. Vogler, D. Montgomery and G. Harper amongst others of the Surface Science Group as US patents 5344611, 5326535, 5320812, 5257633 and 5246666.[15]. BD supports the healthcare industry with market-leading products and services that aim to improve care while lowering costs. Up]Inm$.q[ Q>>Ocm1$'C3+BH&zn7bgRpq$MO^! %PDF-1.3 `Vw)=l]( AxF ;|;J`zqi5/dj An OSHA acceptable, puncture proof containermarked Biohazardous. POLICY: By establishing a procedure for the correct collection of blood by venipuncture many pre-analytical errors and patient management complications can be avoided.
Venous Blood Collection | Services for Health Care Professionals <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>>
BD offers training resources to help improve your clinical practices as part of our goal of advancing the world of health. gr @5c3Ok=3. 2023 BD. The blood separation media takes advantage of the relatively low density of A.
BD 362725 EST Plastic Tube 3ml with Pearlescent White - Ovilcare 21 G x .75 in. 0000015023 00000 n
23 G x .75 in. Tubes must be positively identified after filling with a firmly attached patient label. Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing. Never leave the tourniquet on for over 1 minute. B. /Range[0 1 0 1 0 1 0 1] ESG (Environmental, Social, and Governance), Policies, Guidelines and Statements Center, Our live chat is available between the hours of 8.30am - 5.00pm EST, Monday - Friday, BD Original Equipment Manufacturing (OEM), Patient Care Support Across the Continuum. Blood samples should 2. Assemble needle and syringe, check that it is sharp, unblocked and moving smoothly by passing the air through the syringe.
BD Vacutainer Safety-Lok blood collection set Holding the needle in line with the vein, use a quick, small thrust to penetrate the skin and vein in one motion. 1 0 obj The laboratory will also be responsible for performing assays and reporting results. The Vacutainer Blood Collection Tubes, both plastic and glass, are engineered to reduce specimen spillage.The Safety-Lok Blood Collection Sets and the Eclipse Blood Collection Needles feature a patented .
BD Vacutainer push button blood collection set Disposable, single use needle holder C. Evacuated blood collection tubes D. Tourniquet (non-latex), disposable single use BD and the BD Logo are trademarks of Becton, Dickinson and Company. stream <>>>
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tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Contaminated surfaces must be cleaned with freshly prepared 10% bleach solution. venipuncture site is dry. 7. G. When blood flow stops, remove the tube by holding the hub securely and pulling the tube off the needle.